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Anticorps Monoclonal anti-AHR

AHR Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D1F9

N° de cat : CL488-67785

Synonymes

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated AHR Monoclonal antib (CL488-67785)

    FC experiment of HepG2 using CL488-67785

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human AHR (CL488-67785, Clone:2D1F9) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en cytométriecellules HepG2

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67785 cible AHR dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, rat

Réactivité Humain, rat
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène AHR Protéine recombinante Ag28935
Nom complet aryl hydrocarbon receptor
Masse moléculaire calculée 848 aa, 96 kDa
Poids moléculaire observé105-110 kDa
Numéro d’acquisition GenBankBC070080
Symbole du gène AHR
Identification du gène (NCBI) 196
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Protocole

Product Specific Protocols
FC protocol for CL Plus 488 AHR antibody CL488-67785Download protocol
Standard Protocols
Click here to view our Standard Protocols