Anticorps Monoclonal anti-AGT

AGT Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1B2F5

N° de cat : 68020-1-Ig

Synonymes

AGT, Ang I Angiotensin 2, Ang II Angiotensin 3, Ang III, Angiotensin I, Angiotensin II, Angiotensin III, Angiotensinogen, ANHU, Des Asp[1] angiotensin II, FLJ92595, FLJ97926, Serpin A8, SERPINA8

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Galerie de données de validation

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  WB analysis of human plasma using 68020-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 68020-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig plasma using 68020-1-Ig

  pig plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 68020-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 68020-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 68020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 68020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 68020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 68020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 68020-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AGT antibody (68020-1-Ig, Clone: 1B2F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: plasma humain, cellules HuH-7, cellules THP-1, plasma de porc, tissu placentaire humain
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68020-1-Ig cible AGT dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AGT Protéine recombinante Ag16658
• Nom complet: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Masse moléculaire calculée: 485 aa, 53 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC011519
• Symbole du gène: AGT
• Identification du gène (NCBI): 183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.