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Anticorps Monoclonal anti-AGR2

AGR2 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

IF

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A8A8

N° de cat : CL488-66768

Synonymes

AG 2, AG2, AGR2, GOB 4, HAG 2, HPC8, PDIA17, XAG 2

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated AGR2 Monoclonal anti (CL488-66768)

    IF Staining of human colon cancer using CL488-66768

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 AGR2 antibody (CL488-66768, Clone: 1A8A8 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated AGR2 Monoclonal anti (CL488-66768)

    IF Staining of human colon cancer using CL488-66768

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 AGR2 antibody (CL488-66768, Clone: 1A8A8 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CoraLite® Plus 488-conjugated AGR2 Monoclonal anti (CL488-66768)

    IF Staining of HT-29 using CL488-66768

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite®®488 AGR2 antibody (CL488-66768, Clone: 1A8A8 ) at dilution of 1:200.

Applications testées

Résultats positifs en IFcellules HT-29, tissu de cancer du côlon humain

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66768 cible AGR2 dans les applications de IF et montre une réactivité avec des échantillons Humain, porc

Réactivité Humain, porc
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène AGR2 Protéine recombinante Ag2919
Nom complet anterior gradient homolog 2 (Xenopus laevis)
Masse moléculaire calculée 175 aa, 20 kDa
Poids moléculaire observé 17 kDa
Numéro d’acquisition GenBankBC015503
Symbole du gène AGR2
Identification du gène (NCBI) 10551
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 AGR2 antibody CL488-66768Download protocol
Standard Protocols
Click here to view our Standard Protocols