Anticorps Monoclonal anti-ADRP/Perilipin-2

ADRP/Perilipin-2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G5D4

N° de cat : 60340-1-Ig

Synonymes

ADFP, Adipophilin, ADRP, ADRP/Perilipin 2, Perilipin 2, Perilipin-2, PLIN2

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Galerie de données de validation

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  WB analysis of human adipose using 60340-1-Ig

  human adipose tissue were subjected to SDS PAGE followed by western blot with 60340-1-Ig (ADRP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brown adipose using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu adipeux humain
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu adipeux brun de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60340-1-Ig cible ADRP/Perilipin-2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADRP/Perilipin-2 Protéine recombinante Ag7643
• Nom complet: adipose differentiation-related protein
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 47-52 kDa
• Numéro d’acquisition GenBank: BC005127
• Symbole du gène: ADFP
• Identification du gène (NCBI): 123
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.