Anticorps Monoclonal anti-ADPGK

ADPGK Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2B4B8

N° de cat : 68034-1-Ig

Synonymes

2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35

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Galerie de données de validation

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  WB analysis using 68034-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of LNCaP using 68034-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 68034-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68034-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 68034-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 68034-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, cellules THP-1, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68034-1-Ig cible ADPGK dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADPGK Protéine recombinante Ag31242
• Nom complet: ADP-dependent glucokinase
• Masse moléculaire calculée: 497 aa, 54 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC006112
• Symbole du gène: ADPGK
• Identification du gène (NCBI): 83440
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).