Anticorps Monoclonal anti-Adiponectin receptor 1

• Phare
• Validé par KD/KO

Adiponectin receptor 1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1F12B3

N° de cat : 66619-1-Ig

Synonymes

ACDCR1, Adiponectin receptor 1, Adiponectin receptor protein 1, ADIPOR1, CGI 45, CGI45, PAQR1, TESBP1A

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Galerie de données de validation

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  WB analysis of HEK-293 using 66619-1-Ig

  WB result of Adiponectin receptor 1 antibody (66619-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Adiponectin receptor 1 transfected HEK-293 cells.

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  WB analysis of HeLa using 66619-1-Ig

  HeLa cells, L02 cells, and HepG2 cells were subjected to SDS PAGE followed by western blot with 66619-1-Ig (Adiponectin receptor 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

66619-1-Ig cible Adiponectin receptor 1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Adiponectin receptor 1 Protéine recombinante Ag18187
• Nom complet: adiponectin receptor 1
• Masse moléculaire calculée: 375 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC010743
• Symbole du gène: ADIPOR1
• Identification du gène (NCBI): 51094
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. The human ADIPOR1 gene maps to chromosome 1q32.1, and encodes a 375-amino acid protein with a molecular weight of 43 kDa. This antibody was raised against 1-72aa of human AdipoR1.

Publications

Species	Application	Title
mouse	WB	Food Funct; A flavonoid-rich Smilax china L. extract prevents obesity by upregulating the adiponectin-receptor/AMPK signalling pathway and modulating the gut microbiota in mice.; Authors - Xin Li
mouse	WB	Food Funct; Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling.; Authors - Xiaoling Chen
human	WB	Biol Open; A stem cell based in vitro model of NAFLD enables the analysis of patient specific individual metabolic adaptations in response to a high fat diet and AdipoRon interference.; Authors - Nina Graffmann
pig	WB	Anim Biotechnol; Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway.; Authors - Lu Xiang
pig	WB	Anim Biotechnol; Effect of dietary leucine supplementation on skeletal muscle fiber type transformation in weaning piglets; Authors - Xiaoling Chen
mouse	WB	J Agric Food Chem; Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway; Authors - Chao Tang