Anticorps Monoclonal anti-ADARB1

ADARB1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1H8G3

N° de cat : 67764-1-Ig

Synonymes

ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1

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Galerie de données de validation

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  WB analysis using 67764-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67764-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67764-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67764-1-Ig (ADARB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules 4T1, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67764-1-Ig cible ADARB1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ADARB1 Protéine recombinante Ag17852
• Nom complet: adenosine deaminase, RNA-specific, B1 (RED1 homolog rat)
• Masse moléculaire calculée: 741 aa, 81 kDa
• Poids moléculaire observé: 70 kDa, 80 kDa
• Numéro d’acquisition GenBank: BC065545
• Symbole du gène: ADARB1
• Identification du gène (NCBI): 104
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADARB1 (Double-stranded RNA-specific editase 1) is also named as ADAR2, DRADA2, RED1 and belongs to the ADAR family. It catalyses the deamination of adenosine to inosine at the GluR2 Q/R site in the pre-mRNA encoding the critical subunit of AMPA receptors. This protein has some isoforms with the molecular weight from 77 kDa to 81 kDa and 7 kDa, but it can be detected a very strong band at 50 kDa in addition to a predicted band at 75 kDa as the result of western blot, while ADARB1 has several alternative splice variants (PMID:23103828).