Anticorps Polyclonal de lapin anti-ACE2

• Phare
• Validé par KD/KO

ACE2 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 28868-1-AP

Synonymes

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

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Galerie de données de validation

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  WB analysis using 28868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28868-1-AP (ACE2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28868-1-AP

  WB result of ACE2 antibody (28868-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACE2 transfected HeLa cells.

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  IHC staining of human kidney using 28868-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 28868-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 28868-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 28868-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28868-1-AP (ACE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 28868-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ACE2 antibody (28868-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 28868-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ACE2 antibody (28868-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HeLa, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu rénal humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28868-1-AP cible ACE2 dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACE2 Protéine recombinante Ag30683
• Nom complet: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Masse moléculaire calculée: 805 aa, 92 kDa
• Poids moléculaire observé: 92, 120 kDa
• Numéro d’acquisition GenBank: BC048094
• Symbole du gène: ACE2
• Identification du gène (NCBI): 59272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2.The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088). 28868-1-AP is specific for ACE2, and its antigen region is 30-356aa.