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Anticorps Monoclonal anti-ACC1

ACC1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1A11G10

N° de cat : CL594-67373

Synonymes

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated ACC1 Monoclonal antibody (CL594-67373)

    IF Staining of HepG2 using CL594-67373

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated ACC1 antibody (CL594-67373, Clone: 1A11G10 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated ACC1 Monoclonal antibody (CL594-67373)

    FC experiment of HeLa using CL594-67373

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ACC1 (CL594-67373, Clone:1A11G10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HepG2,
Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67373 cible ACC1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène ACC1 Protéine recombinante Ag17503
Nom complet acetyl-Coenzyme A carboxylase alpha
Masse moléculaire calculée 2383 aa, 275 kDa
Poids moléculaire observé 260-270 kDa
Numéro d’acquisition GenBankBC137287
Symbole du gène ACACA
Identification du gène (NCBI) 31
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410).

Protocole

Product Specific Protocols
IF protocol for CL594 ACC1 antibody CL594-67373Download protocol
Standard Protocols
Click here to view our Standard Protocols