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Anticorps Monoclonal anti-6*His, His-Tag

6*His, His-Tag Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Protéine recombinante

Applications

ELISA

Conjugaison

Biotin

CloneNo.

1B7G5

N° de cat : Biotin-66005

Synonymes

6*His, His-Tag

Galerie de données de validation

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  • ELISA experiment of Recombinant protein using Biotin-conjugated 6*His, His-Tag Monoclonal antibo (Biotin-66005)

    ELISA experiment of Recombinant protein using Biotin-66005

    Biotin-66005 was tested by ELISA. Ag19172(GST-3*MYC-6*HIS-3*FLAG-6*HIS-3*HA-6*HIS)was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of Biotin-66005 (start dilution 1:1000. Bound antibodies were detected with Streptavidin Poly-HRP(1:5000)followed by incubation with HRP Substrate, terminated with 2M H2SO4, then measuring the resulting absorbance at 450 nm.

Applications testées

Résultats positifs en ELISAProtéine recombinante,

Dilution recommandée

ApplicationDilution
Test immuno-enzymatique (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-66005 cible 6*His, His-Tag dans les applications de ELISA et montre une réactivité avec des échantillons Protéine recombinante

Réactivité Protéine recombinante
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet 6*His, His-Tag
Masse moléculaire calculée 1 kDa
Symbole du gène
Identification du gène (NCBI)
Conjugaison Biotin
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.