Anticorps Recombinant de lapin anti-SARS-CoV-2 Nucleocapsid Phosphoprotein

SARS-CoV-2 Nucleocapsid Phosphoprotein Recombinant Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Virus

Applications

WB,ELISA

Conjugaison

Non conjugué

CloneNo.

8C20

N° de cat : 80027-1-RR

Synonymes

2019-nCOV nucleocapsid phosphoprotein, N protein, nucleocapsid phosphoprotein

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Galerie de données de validation

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  WB analysis of Recombinant protein using 80027-1-RR

  E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration.

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  WB analysis of Recombinant protein using 80027-1-RR

  Eukaryotic expressed SARS-CoV-2 Nucleocapsid Phosphoprotein was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration.

• 

  ELISA experiment of Recombinant protein using 80027-1-RR

  Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 80026-1-RR and 80027-1-RR respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

• 

  ELISA experiment of Recombinant protein using 80027-1-RR

  Sandwich ELISA was carried out by coating 80027-1-RR at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated80026-1-RR was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm.

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Applications testées

• Résultats positifs en WB: Phosphoprotéine nucléocapside eucaryote, Ag30676
• Résultats positifs en ELISA: Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Test immuno-enzymatique (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80027-1-RR cible SARS-CoV-2 Nucleocapsid Phosphoprotein dans les applications de WB,ELISA et montre une réactivité avec des échantillons Virus

• Réactivité: Virus
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SARS-CoV-2 Nucleocapsid Phosphoprotein Protéine recombinante Ag30676
• Nom complet: COVID-19 N Protein
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: COVID-19 N Protein
• Identification du gène (NCBI): 43740575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387). A sandwich ELISA for COVID-19 N Protein can be assembled by using 80027-1-RR as capture antibody and conjugated 80026-1-RR for detection.