Anticorps Monoclonal anti-14-3-3E

14-3-3E Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

3A3A4

N° de cat : 66946-1-Ig

Synonymes

14 3 3 protein epsilon, 14 3 3E, FLJ45465, KCIP 1, MDCR, MDS, YWHAE

---

Galerie de données de validation

• 

  WB analysis of HeLa using 66946-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66946-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66946-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66946-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66946-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66946-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66946-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66946-1-Ig cible 14-3-3E dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: 14-3-3E Protéine recombinante Ag25186
• Nom complet: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• Masse moléculaire calculée: 255 aa, 29 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC000179
• Symbole du gène: YWHAE
• Identification du gène (NCBI): 7531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

14-3-3 epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

Publications

Species	Application	Title
human	WB	Cancer Manag Res; miR-31-5p Regulates 14-3-3 ɛ to Inhibit Prostate Cancer 22RV1 Cell Survival and Proliferation via PI3K/AKT/Bcl-2 Signaling Pathway.; Authors - Jiafu Zhao
mouse		Oncol Lett; miR-29b-3p inhibits 22Rv1 prostate cancer cell proliferation through the YWHAE/BCL-2 regulatory axis.; Authors - Jiafu Zhao