SLC7A11/xCT Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC7A11/xCT Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

246

Rezensionen (4)

Kat-Nr. : 26864-1-AP

Synonyme

Amino acid transport system xc, CCBR1, Cystine/glutamate transporter, SLC7A11, SLC7A11/xCT, xCT

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Galerie der Validierungsdaten

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  WB analysis using 26864-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 26864-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 26864-1-AP

  IP result of anti-SLC7A11/xCT(IP:26864-1-AP, 4ug; Detection:26864-1-AP 1:800) with A549 cells lysate 960 ug.

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  IP experiment of HepG2 using 26864-1-AP

  IP result of anti-SLC7A11/xCT(IP:26864-1-AP, 4ug; Detection:26864-1-AP 1:800) with HepG2 cells lysate 1720 ug.

• 

  IHC staining of human renal cell carcinoma using 26864-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 26864-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, BxPC-3-Zellen, HepG2-Zellen, U2OS-Zellen
• Erfolgreiche IP: A549-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 223 publications below
• IHC: See 55 publications below
• IF: See 28 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

26864-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ELISA SLC7A11/xCT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC7A11/xCT fusion protein Ag25431
• Vollständiger Name: solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa, 35-40 kDa
• GenBank-Zugangsnummer: BC012087
• Gene symbol: SLC7A11
• Gene ID (NCBI): 23657
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer. 26864-1-AP recognizes the 55 kDa and 35-40 kDa protein similar to the paper published (PMID: 32188872, 19156441).

Publikationen

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	Cell Host Microbe; Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice; Authors - Yunong Zeng
human	WB	Nat Immunol; Glutathione peroxidase 4-regulated neutrophil ferroptosis induces systemic autoimmunity.; Authors - Pengchong Li
human	WB	ACS Nano; Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy; Authors - Rangrang Fan
human	WB	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
human	WB	Nat Chem Biol; Hydropersulfides inhibit lipid peroxidation and ferroptosis by scavenging radicals; Authors - Uladzimir Barayeu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chloe (Verified Customer) (11-01-2023)

Great antibody works pretty well for a western blot with 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Breast Cancer Cell Lines

SLC7A11/xCT Antibody Western Blot validation (1:1000 dilution) in Breast Cancer Cell Lines (Cat no:26864-1-AP)

FH

James (Verified Customer) (11-18-2022)

Detection of SLC7A11 in liver protein extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

SLC7A11/xCT Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:26864-1-AP)

FH

Melanie (Verified Customer) (05-11-2022)

Tested the antibody in Hek293T cells. Had to dilute the antibody 1:50000, since the signal was to strong. Very clear gang at the calculated MW. Good antibody!

• Applications: Western Blot
• Cell Tissue Type: Hek293T

FH

Amélie (Verified Customer) (05-03-2022)

We used the AB on Bone Marrow derived Macrophages, where Slc7a11 is KO (validate by RT PCR), in Western blot. We did not found a specific staining in the right size.

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: Bone Marrow derived macrophages from WT or KO mice

SLC7A11/xCT Antibody Western Blot validation (1000 dilution) in Bone Marrow derived macrophages from WT or KO mice (Cat no:26864-1-AP)