transgelin/SM22 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

transgelin/SM22 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-10493

Synonyme

22 kDa actin binding protein, DKFZp686P11128, Protein WS3 10, SM22, SM22 alpha, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1, WS3 10

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Galerie der Validierungsdaten

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  IF Staining of mouse heart using CL594-10493

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 transgelin/SM22 antibody (CL594-10493) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Mausherzgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-10493 bindet in IF transgelin/SM22 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: transgelin/SM22 fusion protein Ag0764
• Vollständiger Name: transgelin
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 19-22 kDa
• GenBank-Zugangsnummer: BC004927
• Gene symbol: TAGLN
• Gene ID (NCBI): 6876
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The transgelin family is a group of proteins that belong to 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgelin 1 protein. It can cross-react with other two transgelins based on the sequence similarity.