SARS-CoV-2 S protein (428-506 aa) Polyklonaler Antikörper

SARS-CoV-2 S protein (428-506 aa) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 28901-1-AP

Synonyme

SARS-CoV-2 Spike Glycoprotein (428-506 aa)

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Galerie der Validierungsdaten

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  WB analysis using 28901-1-AP

  Spike Glycoprotein with the domain 1-658aa expressed in HEK-293 cells were subjected to SDS PAGE followed by western blot with 28901-1-AP (SARS-CoV-2 Spike Glycoprotein (428-506 aa) Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  ELISA experiment of Recombinant protein using 28901-1-AP

  SARS-CoV-2 Spike Glycoprotein (428-506aa) Antibody (28901-1-AP) tested by ELISA.SARS-CoV-2 Spike protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Glycoprotein (428-506aa) Antibody (28901-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in ELISA: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28901-1-AP bindet in WB, ELISA SARS-CoV-2 S protein (428-506 aa) und zeigt Reaktivität mit Virus

• Getestete Reaktivität: Virus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SARS-CoV-2 S protein (428-506 aa) fusion protein Ag30684
• Vollständiger Name: SARS-CoV-2 Spike Protein
• Berechnetes Molekulargewicht: 141 kDa
• GenBank-Zugangsnummer: NC_045512
• Gene symbol: COVID-19 S Protein
• Gene ID (NCBI): 43740568
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Irene (Verified Customer) (10-15-2020)

Tested the antibody for IF and western blot and it did not work for either application. There were minimal colocalization but also non-specific binding for IF.

• Applications: Western Blot, Immunofluorescence