smooth muscle actin Polyklonaler Antikörper

smooth muscle actin Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen

1

Kat-Nr. : CL594-14395

Synonyme

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, smooth muscle actin

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Galerie der Validierungsdaten

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  IF Staining of mouse heart using CL594-14395

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin antibody (CL594-14395) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL594-14395

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite®594 smooth muscle actin antibody (CL594-14395) at dilution of 1:200, CoraLite® Plus 488 ZG16 antibody (CL488-67389, Clone: 1A7B9, green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Mausherzgewebe, Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-14395 bindet in IF smooth muscle actin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: smooth muscle actin fusion protein Ag5886
• Vollständiger Name: actin, alpha 2, smooth muscle, aorta
• Berechnetes Molekulargewicht: 377 aa, 42 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC017554
• Gene symbol: ACTA2
• Gene ID (NCBI): 59
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Publikationen

Species	Application	Title
	IF	Front Pharmacol; Pan-cancer analysis identifies NT5E as a novel prognostic biomarker on cancer-associated fibroblasts associated with unique tumor microenvironment; Authors - Xin-Miao Xue