Phospho-TDP43 (Ser403/404) Monoklonaler Antikörper

Phospho-TDP43 (Ser403/404) Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

6B11B12

Rezensionen (1)

Kat-Nr. : 66079-1-Ig

Synonyme

ALS10, Phospho-TDP43 (Ser403/404), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

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Galerie der Validierungsdaten

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  WB analysis using 66079-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of K-562 using 66079-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66079-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66079-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66079-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Jurkat using 66079-1-Ig

  1X10^6 Jurkat cells were stained with 0.20ug Phospho-TDP43 (Ser403/404) antibody (66079-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, fetales humanes Hirngewebe, Jurkat-Zellen, K-562-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

66079-1-Ig bindet in WB, IHC, IF, FC, ELISA Phospho-TDP43 (Ser403/404) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TAR DNA binding protein
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_007375
• Gene symbol: TARDBP
• Gene ID (NCBI): 23435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).66079-1-Ig is a mouse monoclonal antibody recognizing TDP-43 only when phosphorylated at 403/404. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Publikationen

Species	Application	Title
human,zebrafish	IHC,IF	Nat Neurosci; TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury; Authors - Alessandro Zambusi
human	IHC	Acta Neuropathol Commun; Influence of APOE genotype in primary age-related tauopathy.; Authors - Andrew C Robinson
human	WB	Eur J Med Chem; Targeting nuclear protein TDP-43 by cell division cycle kinase 7 inhibitors: A new therapeutic approach for amyotrophic lateral sclerosis.; Authors - Elisa Rojas-Prats
human	IF	Biochemistry; Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.; Authors - Janice S W Ng
human	WB	PLoS One; Drosophila lines with mutant and wild type human TDP-43 replacing the endogenous gene reveals phosphorylation and ubiquitination in mutant lines in the absence of viability or lifespan defects.; Authors - Jer-Cherng Chang
human	WB	Methods Mol Biol; Sedimentation Assays to Assess the Impact of Posttranslational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells; Authors - Lara A Gruijs da Silva

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by Phospho-TDP43 (Ser403/404) Monoclonal antibody at dilution of 1:100 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: NSC-34 cells