pan Ras Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

pan Ras Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

4H4G7

Kat-Nr. : CL555-60309

Synonyme

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2A, KRAS2B, KRAS2B speicifc, Krasa, Krasb, NS3, RASK2

---

Galerie der Validierungsdaten

• 

  FC experiment of HeLa using CL555-60309

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human pan Ras (CL555-60309, Clone:4H4G7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-60309 bindet in FC (Intra) pan Ras und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: pan Ras fusion protein Ag2700
• Vollständiger Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 188 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC013572
• Gene symbol: KRAS
• Gene ID (NCBI): 3845
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C