Pan-PAX Polyklonaler Antikörper

Pan-PAX Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 21383-1-AP

Synonyme

paired box, paired box 8, Paired box protein Pax, Paired box protein Pax 8, Pan-PAX, PAX 8

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Galerie der Validierungsdaten

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  WB analysis of SKOV-3 using 21383-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 21383-1-AP (Pan-PAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 21383-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21383-1-AP (Pan-PAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SKOV-3 using 21383-1-AP

  IP Result of anti-pan-PAX (IP:21383-1-AP, 3ug; Detection:21383-1-AP 1:800) with SKOV-3 cells lysate 2500ug.

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  IHC staining of human ovary tumor using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SKOV-3 using 21383-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using Pan-PAX antibody (21383-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, A2780-Zellen
• Erfolgreiche IP: SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Nierengewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

21383-1-AP bindet in WB, IP, IHC, IF, ELISA Pan-PAX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Pan-PAX fusion protein Ag16018
• Vollständiger Name: paired box 8
• Berechnetes Molekulargewicht: 450 aa, 48 kDa
• Beobachtetes Molekulargewicht: 36-48 kDa
• GenBank-Zugangsnummer: BC001060
• Gene symbol: PAX8
• Gene ID (NCBI): 7849
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX genes encode a family of developmentally regulated transcription factors that have been implicated in a number of human and murine congenital disorders, as well as in tumorigenesis. These genes are defined by the presence of an evolutionarily conserved DNA binding domain, termed the paired domain. Many paired domain-containing proteins recognize a common sequence motif. The immunogen of this atnibody is the Paired box domain of PAX family. This antibody is a Pan-PAX antibody.

Publikationen

Species	Application	Title
mouse	IF	Life Sci; Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy.; Authors - Qiwang Ma
rat	IHC	Sci Signal; Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.; Authors - Zi-Yang Zhang
human	IHC	Am J Surg Pathol; PAX8 and PAX2 immunostaining facilitates the diagnosis of primary epithelial neoplasms of the male genital tract.; Authors - Tong Guo-Xia GX
mouse	IHC	Endocrine; PMID: 32281047; Authors - Mika Shimamura
pig	IF	PLoS One; PMID: 28231273; Authors - Sergio E Palma-Vera