p57Kip2 Polyklonaler Antikörper

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• KD/KO Validated

p57Kip2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 23317-1-AP

Synonyme

BWCR, BWS, CDKN1C, KIP2, p57, p57Kip2, WBS

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Galerie der Validierungsdaten

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  WB analysis using 23317-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23317-1-AP (p57Kip2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 23317-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23317-1-AP (p57Kip2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 23317-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23317-1-AP (p57Kip2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 23317-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23317-1-AP (p57Kip2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 23317-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23317-1-AP (p57Kip2 antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

23317-1-AP bindet in WB, IHC, CoIP, ELISA p57Kip2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: p57Kip2 fusion protein Ag19849
• Vollständiger Name: cyclin-dependent kinase inhibitor 1C (p57, Kip2)
• Berechnetes Molekulargewicht: 316 aa, 32 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC067842
• Gene symbol: CDKN1C
• Gene ID (NCBI): 1028
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1C, also named as p57KIP2 and KIP2, belongs to the CDI family. CDKN1C is a potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. It is a negative regulator of cell proliferation. CDKN1C may play a role in maintenance of the non-proliferative state throughout life.

Publikationen

Species	Application	Title
human	WB	Clin Cancer Res; microRNA-221 Enhances MYCN via Targeting Nemo-like Kinase and Functions as an Oncogene Related to Poor Prognosis in Neuroblastoma.; Authors - Xiao-Yan He
human	WB	FASEB J; Downregulation of ALKBH5 rejuvenates aged human mesenchymal stem cells and enhances their therapeutic efficacy in myocardial infarction; Authors - Xiaoyan Gao
human	WB,IHC	Cancer Med; PIK3R3 is upregulated in liver cancer and activates Akt signaling to control cancer growth by regulation of CDKN1C and SMC1A; Authors - Weidong Lin ; KD Validated
human	WB,CoIP,IHC	Cell Death Dis; The FOXM1/RNF26/p57 axis regulates the cell cycle to promote the aggressiveness of bladder cancer.; Authors - Lu Yi
human	WB	Oncogene; Dual network analysis of transcriptome data for discovery of new therapeutic targets in non-small cell lung cancer; Authors - Yuquan Bai