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p130Cas / BCAR1 Polyklonaler Antikörper

p130Cas / BCAR1 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-16815

Synonyme

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated p130Cas / BCAR1 Polyclonal (CL555-16815)

    IF Staining of HeLa using CL555-16815

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 p130Cas / BCAR1 antibody (CL555-16815) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®555-conjugated p130Cas / BCAR1 Polyclonal (CL555-16815)

    FC experiment of A431 using CL555-16815

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human p130Cas / BCAR1 (CL555-16815) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCA431-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-16815 bindet in IF, FC (Intra) p130Cas / BCAR1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen p130Cas / BCAR1 fusion protein Ag10354
Vollständiger Name breast cancer anti-estrogen resistance 1
Berechnetes Molekulargewicht 870 aa, 93 kDa
Beobachtetes Molekulargewicht 116 kDa
GenBank-ZugangsnummerBC062556
Gene symbol BCAR1
Gene ID (NCBI) 9564
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
IF protocol for CL555 p130Cas / BCAR1 antibody CL555-16815Protokoll herunterladen
FC protocol for CL555 p130Cas / BCAR1 antibody CL555-16815Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen