p115, USO1 Monoklonaler Antikörper

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• KD/KO Validated

p115, USO1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B7D8

Kat-Nr. : 68100-1-Ig

Synonyme

P115, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 68100-1-Ig

  WB result of p115, USO1 antibody (68100-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p115, USO1 transfected HeLa cells.

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  WB analysis using 68100-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68100-1-Ig (p115, USO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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  IHC staining of human liver cancer using 68100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68100-1-Ig (p115, USO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 68100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68100-1-Ig (p115, USO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 68100-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (68100-1-Ig, Clone: 3B7D8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68100-1-Ig bindet in WB, IHC, IF, ELISA p115, USO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: p115, USO1 fusion protein Ag5543
• Vollständiger Name: USO1 homolog, vesicle docking protein (yeast)
• Berechnetes Molekulargewicht: 962 aa, 108 kDa
• Beobachtetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: BC032654
• Gene symbol: USO1
• Gene ID (NCBI): 8615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).