Nectin-4/PVRL4 Polyklonaler Antikörper

Nectin-4/PVRL4 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 21903-1-AP

Synonyme

Ig superfamily receptor LNIR, LNIR, nectin 4, Nectin-4, poliovirus receptor related 4, PRR4, PVRL4

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 21903-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21903-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21903-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 21903-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 21903-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21903-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 21903-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 21903-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Nectin-4/PVRL4 antibody (21903-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A2780-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

21903-1-AP bindet in WB, IHC, IF, FC, ELISA Nectin-4/PVRL4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nectin-4/PVRL4 fusion protein Ag14127
• Vollständiger Name: poliovirus receptor-related 4
• Berechnetes Molekulargewicht: 510 aa, 55 kDa
• Beobachtetes Molekulargewicht: 43 kDa, 66 kDa
• GenBank-Zugangsnummer: BC010423
• Gene symbol: PVRL4
• Gene ID (NCBI): 81607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

Publikationen

Species	Application	Title
human	WB	Mol Oncol; MiR-31 and miR-128 regulates poliovirus receptor-related 4 mediated measles virus infectivity in tumors.; Authors - Hirosha Geekiyanage
mouse	WB,IHC	Int J Urol; Chemotherapy with gemcitabine and cisplatin downregulates tumor expression level of nectin-4 in a syngeneic model of murine MBT2 urothelial cancer cell line and C3H mice; Authors - Makito Miyake
human	IHC	Oncol Lett; Upregulation of nectin-4 is associated with ITGB1 and vasculogenic mimicry and may serve as a predictor of poor prognosis in colorectal cancer.; Authors - Jinxiu Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Morgan (Verified Customer) (08-09-2021)

I had a lot of trouble using this PVRL4 antibody on western blots of 2 ovarian cancer cell lines; OV8 and OV90. I kept getting prominent bands at 250 kda. I tried cutting the 250 band to see if that would help, but it did not make the signal any better in the band(s) below. Any insight on this would be greatly appreciated. Thank you.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: OV8 and 0V90

Nectin-4/PVRL4 Antibody Western Blot validation (1:1000 dilution) in OV8 and 0V90 (Cat no:21903-1-AP)