m6A Monoklonaler Antikörper

Name: m6A Mouse Monoclonal antibody
Brand: Proteintech
SKU: 68055-1-Ig
Rating: 5.00 (2 reviews)

m6A Monoklonal Antikörper für Dot Blot, IHC, RIP, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human, m6A, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, Dot Blot, ELISA

Konjugation

Unkonjugiert

Publikationen

CloneNo.

1D5E10

Rezensionen (2)

Kat-Nr. : 68055-1-Ig

Synonyme

6-N-Methyladenoside, m6A, N6-methyladenosine

Galerie der Validierungsdaten

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AllRIPIHCELISADot Blot

RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDF1(m6A reader) antibody 17479-1-AP (1:2000). (Lysate: 3.6mg per IP; IP: 15µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDF1(m6A reader) antibody 17479-1-AP (1:2000). (Lysate: 3.6mg per IP; IP: 15µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with IGF2BP3 (m6A reader) antibody 66526-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with IGF2BP3 (m6A reader) antibody 66526-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with FMR1 (m6A reader) antibody 66548-1-Ig (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with FMR1 (m6A reader) antibody 66548-1-Ig (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDF1 (m6A reader) antibody 66745-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDF1 (m6A reader) antibody 66745-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with FMR1 (m6A reader) antibody 13755-1-AP (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with FMR1 (m6A reader) antibody 13755-1-AP (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDF2 (m6A reader) antibody 24744-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDF2 (m6A reader) antibody 24744-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDC2 (m6A reader) antibody 27779-1-Ig (1:4000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDC2 (m6A reader) antibody 27779-1-Ig (1:4000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with LRPPRC (m6A reader) antibody 21175-1-AP (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with LRPPRC (m6A reader) antibody 21175-1-AP (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDF3 (m6A reader) antibody 25537-1-Ig (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDF3 (m6A reader) antibody 25537-1-Ig (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with HNRNPA2B1 (m6A reader) antibody 67445-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with HNRNPA2B1 (m6A reader) antibody 67445-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with HuR (m6A reader) antibody 11910-1-AP (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with HuR (m6A reader) antibody 11910-1-AP (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with HuR (m6A reader) antibody 66549-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with HuR (m6A reader) antibody 66549-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with IGF2BP1 (m6A reader) antibody 22803-1-AP (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with IGF2BP1 (m6A reader) antibody 22803-1-AP (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with IGF2BP2 (m6A reader) antibody 11601-1-AP (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with IGF2BP2 (m6A reader) antibody 11601-1-AP (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
RIP experiment of RNA using 68055-1-Ig
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with IGF2BP3 (m6A reader) antibody 14642-1-AP (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
×
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with IGF2BP3 (m6A reader) antibody 14642-1-AP (1:5000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
IHC staining of mouse testis using 68055-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat lymph node using 68055-1-Ig
Immunohistochemical analysis of paraffin-embedded rat lymph node slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat lymph node using 68055-1-Ig
Immunohistochemical analysis of paraffin-embedded rat lymph node slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 68055-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 68055-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 68055-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 68055-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 68055-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 68055-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 68055-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ELISA experiment of m6A using 68055-1-Ig
Indirect ELISA and competitive ELISA results show that this antibody is specific to m6A. Indirect ELISA was performed by coating BSA conjugated m6A at 20ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of m6A or its structure analogue compounds are mixed in primary antibody (fixed at 0.05μg/mL).
×
Indirect ELISA and competitive ELISA results show that this antibody is specific to m6A. Indirect ELISA was performed by coating BSA conjugated m6A at 20ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of m6A or its structure analogue compounds are mixed in primary antibody (fixed at 0.05μg/mL).
Dot Blot experiment of RNA using 68055-1-Ig
Total RNA was isolated from HEK-293 cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with BSA and blotted with m6A antibody 68055-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using unrelated antibody with the same isotype (UCP2 antibody 66700-1-Ig) at the same dose.
×
Total RNA was isolated from HEK-293 cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with BSA and blotted with m6A antibody 68055-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using unrelated antibody with the same isotype (UCP2 antibody 66700-1-Ig) at the same dose.

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Geprüfte Anwendungen

Erfolgreiche Detektion in RIP	RNA
Erfolgreiche Detektion in IHC	Maushodengewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe **Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. () Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.***
Erfolgreiche Detektion in ELISA	m6A
Erfolgreiche Detektion in Dot Blot	RNA

Empfohlene Verdünnung

Anwendung	Verdünnung
RImmunpräzipitation (IP)	RIP : 1:1000-1:4000
Immunhistochemie (IHC)	IHC : 1:2000-1:8000
Enzyme-linked Immunosorbent Assay (ELISA)	ELISA : 1:2000-1:20000
DOT BLOT	DOT BLOT : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WB	See 3 publications below
IHC	See 3 publications below
IF	See 1 publications below
IP	See 1 publications below
RIP	See 6 publications below

Produktinformation

68055-1-Ig bindet in WB, RIP, IP, IHC, IF, Dot Blot, ELISA m6A und zeigt Reaktivität mit human, m6A, Maus, Ratten

Getestete Reaktivität	human, m6A, Maus, Ratte
In Publikationen genannte Reaktivität	human, Hausschwein, Maus, Ratte
Wirt / Isotyp	Maus / IgG3
Klonalität	Monoklonal
Typ	Antikörper
Immunogen	Verbindung
Vollständiger Name	m6A
GenBank-Zugangsnummer	m6A
Gene symbol
Gene ID (NCBI)
Konjugation	Unkonjugiert
Form	Liquid
Reinigungsmethode	Protein-A-Reinigung
Lagerungspuffer	PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
Lagerungsbedingungen	Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

m6A (N6-methyladenosine) is the most abundant internal modification in mammalian mRNA. This modification is installed by the m6A methyltransferases or termed "writers"such as METTL3 and METTL14, and can be reversed by demethylases that serve as "erasers" such as FTO and ALKBH5. The stability of m6A-modified mRNA is regulated by m6A reader protein YTHDFs, which recognizes m6A and reduces the stability of target transcripts. m6A modification and its regulatory proteins play critical roles in cancer pathogenesis and progression. m6A modification is also invovled in viruses life cycles, suggesting that drugs targets to m6A pathway could be used for antiviral thereapy.

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68055-1-IgDotBlot.pdf

Protokolle

Produktspezifische Protokolle
IHC protocol for m6A antibody 68055-1-Ig	Protokoll herunterladen

Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

Species	Application	Title
	RIP	Oncogene N6-methyladenosine reader YTHDF2 promotes multiple myeloma cell proliferation through EGR1/p21cip1/waf1/CDK2-Cyclin E1 axis-mediated cell cycle transition Authors - Rui Liu View Article
mouse	IHC	Cells Aberrant DNA and RNA Methylation Occur in Spinal Cord and Skeletal Muscle of Human SOD1 Mouse Models of ALS and in Human ALS: Targeting DNA Methylation Is Therapeutic Authors - Lee J Martin View Article
human		Front Genet Transcriptome-Wide Analysis of RNA N6-Methyladenosine Modification in Adriamycin-Resistant Acute Myeloid Leukemia Cells. Authors - Shu Fang View Article
human	WB	Cell Death Discov Targeting AKT induced Ferroptosis through FTO/YTHDF2-dependent GPX4 m6A methylation up-regulating and degradating in colorectal cancer Authors - Ge Zhang View Article
		Nan Fang Yi Ke Da Xue Xue Bao [m6A methylase WTAP participates in renal ischemia-reperfusion injury by regulating FOXO1 expression] Authors - G Zhao View Article
	IP,RIP	FASEB J FTO-mediated m6A modification of serum amyloid A2 mRNA promotes podocyte injury and inflammation by activating the NF-κB signaling pathway Authors - Yating Lang View Article

Rezensionen

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Tatyana (Verified Customer) (12-04-2023)	Used for dot blot on total RNA, strong signal Applications: Other Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human / SH-SY5Y cells m6A Antibody Other validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:68055-1-Ig)
FH H (Verified Customer) (08-02-2022)	This antibody works great in Human cells lines Applications: Immunoprecipitation Cell Tissue Type: HCT15

m6A Monoklonaler Antikörper

m6A Monoklonal Antikörper für Dot Blot, IHC, RIP, ELISA

Galerie der Validierungsdaten

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

RIP experiment of RNA using 68055-1-Ig

IHC staining of mouse testis using 68055-1-Ig

IHC staining of rat lymph node using 68055-1-Ig

IHC staining of rat lymph node using 68055-1-Ig

IHC staining of human lung cancer using 68055-1-Ig

IHC staining of human lung cancer using 68055-1-Ig

IHC staining of human breast cancer using 68055-1-Ig

IHC staining of human breast cancer using 68055-1-Ig

IHC staining of human colon cancer using 68055-1-Ig

IHC staining of human colon cancer using 68055-1-Ig

IHC staining of mouse testis using 68055-1-Ig

ELISA experiment of m6A using 68055-1-Ig

Dot Blot experiment of RNA using 68055-1-Ig

Geprüfte Anwendungen

Empfohlene Verdünnung

Veröffentlichte Anwendungen

Produktinformation

Hintergrundinformationen

Protokolle

Publikationen

N6-methyladenosine reader YTHDF2 promotes multiple myeloma cell proliferation through EGR1/p21cip1/waf1/CDK2-Cyclin E1 axis-mediated cell cycle transition

Aberrant DNA and RNA Methylation Occur in Spinal Cord and Skeletal Muscle of Human SOD1 Mouse Models of ALS and in Human ALS: Targeting DNA Methylation Is Therapeutic

Transcriptome-Wide Analysis of RNA N6-Methyladenosine Modification in Adriamycin-Resistant Acute Myeloid Leukemia Cells.

Targeting AKT induced Ferroptosis through FTO/YTHDF2-dependent GPX4 m6A methylation up-regulating and degradating in colorectal cancer

[m6A methylase WTAP participates in renal ischemia-reperfusion injury by regulating FOXO1 expression]

FTO-mediated m6A modification of serum amyloid A2 mRNA promotes podocyte injury and inflammation by activating the NF-κB signaling pathway

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