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Gamma Tubulin Monoklonaler Antikörper

Gamma Tubulin Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3F9H8

Kat-Nr. : CL594-66320

Synonyme

Gamma 1 tubulin, Gamma Tubulin, GCP 1, TUBG, TUBG1, TUBGCP1, Tubulin gamma 1 chain, tubulin, gamma 1

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite®594-conjugated Gamma Tubulin Monoclonal a (CL594-66320)

    IF Staining of MDCK using CL594-66320

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CL594-66320 (gamma tubulin antibody) at dilution of 1:50.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Gamma Tubulin Monoclonal a (CL594-66320)

    FC experiment of HeLa using CL594-66320

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Gamma Tubulin (CL594-66320, Clone:3F9H8) (red), or 0.8 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMDCK-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66320 bindet in IF, FC (Intra) Gamma Tubulin und zeigt Reaktivität mit human, Hund, Maus, Ratten

Getestete Reaktivität human, Hund, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen Gamma Tubulin fusion protein Ag23913
Vollständiger Name tubulin, gamma 1
Berechnetes Molekulargewicht 51 kDa
Beobachtetes Molekulargewicht 48-55 kDa
GenBank-ZugangsnummerBC000619
Gene symbol TUBG1
Gene ID (NCBI) 7283
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 Gamma Tubulin antibody CL594-66320Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen