GFP tag Polyklonaler Antikörper

GFP tag Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

aequorea victoria, rekombinanten Protein und mehr (3)

Anwendung

WB, RIP, IP-MS, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

565

Rezensionen (11)

Kat-Nr. : 50430-2-AP

Synonyme

CFP, eGFP, eYFP, GFP, GFP tag, YFP

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Galerie der Validierungsdaten

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  WB analysis of recombinant protein using 50430-2-AP

  Western blot of eGFP-tagged fusion protein with anti-eGFP-tag (50430-2-AP) at various dilutions.

• 

  WB analysis of Transfected HEK-293 using 50430-2-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 50430-2-AP (GFP tag Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Transfected HEK-293 using 50430-2-AP

  IP Result of anti-GFP tag (IP:50430-2-AP, 4ug; Detection:50430-2-AP 1:5000) with Transfected HEK-293 cells lysate 300ug.

• 

  IF Staining of Transfected HEK-293 using 50430-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed Transfected HEK-293 cells using 50430-2-AP (GFP tag antibody) at dilution of 1:100 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Transfected HEK-293 using 50430-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody (50430-2-AP) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: recombinant protein, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF: Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 301 publications below
• IHC: See 13 publications below
• IF: See 119 publications below
• IP: See 51 publications below
• CoIP: See 27 publications below
• ChIP: See 3 publications below
• RIP: See 1 publications below

Produktinformation

50430-2-AP bindet in WB, RIP, IP-MS, IP, IHC, IF, CoIP, ChIP, ELISA GFP tag und zeigt Reaktivität mit aequorea victoria, rekombinanten Protein

• Getestete Reaktivität: aequorea victoria, rekombinanten Protein
• In Publikationen genannte Reaktivität: Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFP tag fusion protein Ag2128
• Vollständiger Name: GFP tag
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: U73901
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.

Publikationen

Species	Application	Title
	WB	Cell; Phase separation drives RNA virus-induced activation of the NLRP6 inflammasome.; Authors - Chen Shen
	WB,IF	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang
	WB	Nat Genet; Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.; Authors - Margot A Cousin
	IF	Cell Stem Cell; DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State.; Authors -
	WB	Nat Methods; TALEN-mediated precise genome modification by homologous recombination in zebrafish.; Authors - Zu Yao Y
	IF	Immunity; Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.; Authors - Zhen Xiong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (05-26-2023)

Excellent

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 500
• Cell Tissue Type: AC16

GFP tag Antibody Western Blot,Immunofluorescence validation (500 dilution) in AC16 (Cat no:50430-2-AP)

FH

PK (Verified Customer) (03-20-2023)

Very Good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEK

GFP tag Antibody Western Blot validation (1000 dilution) in HEK (Cat no:50430-2-AP)

FH

Stephen (Verified Customer) (08-02-2022)

HeLa cells transfected with plasmid expressing EGFP fixed with 4% PFA for 15 mins and immunostained with rabbit GFP antibody at 4 degrees incubated for 20h washed then incubated secondary alexa 594 and DAPI for 1 hour room temp imaged on wide field fluorescence microscope.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

GFP tag Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:50430-2-AP)

FH

A (Verified Customer) (01-04-2022)

Works for IP

• Applications: Immunoprecipitation
• Cell Tissue Type: C. elegans

FH

Rinalda (Verified Customer) (06-21-2021)

Great product

• Applications: Western Blot
• Cell Tissue Type: fungal cells

FH

Lana (Verified Customer) (12-22-2020)

SDS-PAGE: 15 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) for 2h at 80V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:2500.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 – HEK293 whole cell lysate, negative transfection, 3 – whole cell lysate of cells transfected with eGFP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: HEK293

GFP tag Antibody Western Blot validation (1:2500 dilution) in HEK293 (Cat no:50430-2-AP)

FH

Paul (Verified Customer) (01-15-2020)

Works well for WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Laura (Verified Customer) (01-14-2020)

Good sensitivity for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Aamir (Verified Customer) (01-08-2020)

Works well for WB and IF

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEKs

FH

Erica (Verified Customer) (09-26-2019)

This antibody works very well with both western blots and IP. We previously used GFP antibody from another company and it didn't work well. We then switched to Proteinntech's and the signal was very strong! Highly recommend!

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000 WB
• Cell Tissue Type: HEK, MEF, HELA

FH

Mayur (Verified Customer) (04-30-2019)

The H4 cells were transfected with pcDNA only and pcDNA EGFP . The expression of the EGFP was measured using the GFP antibody from ProteinTech. Great antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5K
• Cell Tissue Type: H4 cells

GFP tag Antibody Western Blot, validation (1:5K dilution) in H4 cells (Cat no:50430-2-AP)