c-SRC Polyklonaler Antikörper

c-SRC Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 25978-1-AP

Synonyme

ASV, c SRC, c-SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

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Galerie der Validierungsdaten

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  WB analysis of A431 using 25978-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25978-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of CHO using 25978-1-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25978-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 25978-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25978-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25978-1-AP (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25978-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25978-1-AP (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, CHO-Zellen, COLO 320-Zellen, Jurkat-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

25978-1-AP bindet in WB, IHC, ELISA c-SRC und zeigt Reaktivität mit hamster, human, Maus

• Getestete Reaktivität: hamster, human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: c-SRC fusion protein Ag22415
• Vollständiger Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC011566
• Gene symbol: SRC
• Gene ID (NCBI): 6714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

Publikationen

Species	Application	Title
human	WB	Life Sci; Design and screening of a novel neuropilin-1 targeted penetrating peptide for anti-angiogenic therapy in glioma.; Authors - Lin Zhao
human	WB	Oncotarget; Targeting claudin-4 enhances CDDP-chemosensitivity in gastric cancer.; Authors - Yukiko Nishiguchi