APP Polyklonaler Antikörper

APP Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 27320-1-AP

Synonyme

A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, APP, APPI, beta Amyloid, CTFgamma, CVAP, Gamma CTF(50) C31, PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta

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Galerie der Validierungsdaten

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  WB analysis using 27320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27320-1-AP (beta Amyloid antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27320-1-AP (APP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27320-1-AP (APP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 27320-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 27320-1-AP (beta Amyloid antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, HEK-293T-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27320-1-AP bindet in WB, IHC, ELISA APP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APP fusion protein Ag26299
• Vollständiger Name: amyloid beta (A4) precursor protein
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC004369
• Gene symbol: APP
• Gene ID (NCBI): 351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. APP can be cleaved into several chains, this antibody could recognize N-terminal fragment of APP (N-APP).

Publikationen

Species	Application	Title
human	WB	Alzheimers Res Ther; Krüppel-like factor 5 accelerates the pathogenesis of Alzheimer's disease via BACE1-mediated APP processing.; Authors - Yaqi Wang