Beta Actin Monoklonaler Antikörper

Beta Actin Monoklonal Antikörper für IF, WB

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Hund, Maus, Pflanze, Ratte, Zebrafisch

Anwendung

WB, IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

7D2C10

Kat-Nr. : CL594-60008

Synonyme

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

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Galerie der Validierungsdaten

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  WB analysis using CL594-60008

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-60008 (Beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using CL594-60008

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.

• 

  IF Staining of MDCK using CL594-60008

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60008 bindet in WB, IF Beta Actin und zeigt Reaktivität mit human, Hund, Maus, Pflanze, Ratten, Zebrafisch

• Getestete Reaktivität: human, Hund, Maus, Pflanze, Ratte, Zebrafisch
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Beta Actin fusion protein Ag0297
• Vollständiger Name: actin, beta
• Berechnetes Molekulargewicht: 375 aa, 42 kDa
• GenBank-Zugangsnummer: BC002409
• Gene symbol: ACTB
• Gene ID (NCBI): 60
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody is conjugated with CL594,Ex/Em 593 nm/614 nm.