Beta Catenin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Beta Catenin Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

105

Kat-Nr. : 17565-1-AP

Synonyme

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin

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Galerie der Validierungsdaten

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  WB analysis using 17565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17565-1-AP (Beta Catenin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17565-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17565-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of MCF-7 using 17565-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse liver using 17565-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17565-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17565-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17565-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 17565-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human liver cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 17565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).

• 

  IF Staining of MCF-7 using 17565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).

• 

  IF Staining of MCF-7 using 17565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of T-47D using 17565-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  FC experiment of MCF-7 using 17565-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Beta Catenin (17565-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, C6-Zellen, HeLa-Zellen, Maushirngewebe, Mauslebergewebe, MCF-7-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 85 publications below
• IHC: See 17 publications below
• IF: See 29 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

17565-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA Beta Catenin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Beta Catenin fusion protein Ag11763
• Vollständiger Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Berechnetes Molekulargewicht: 781 aa, 86 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC058926
• Gene symbol: CTNNB1
• Gene ID (NCBI): 1499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Publikationen

Species	Application	Title
mouse	WB,IF	J Clin Invest; JAM3 maintains leukemia-initiating cell self-renewal through LRP5/AKT/β-catenin/CCND1 signaling.; Authors - Yaping Zhang
human	WB,IHC,IF,IP	Cell Death Dis; USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization; Authors - Jianing Tang
rat	WB,IF	Mater Today Bio; A novel adhesive dual-sensitive hydrogel for sustained release of exosomes derived from M2 macrophages promotes repair of bone defects; Authors - Xiaoying Zhao
human	IHC,IF	Br J Pharmacol; Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1.; Authors - Feng Zhang
human	WB	Acta Pharmacol Sin; Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction.; Authors - Hai-Yan Wang
human		Int J Nanomedicine; Self-Assembly Of Retinoid Nanoparticles For Melanoma Therapy.; Authors - Han Liao