Alpha Synuclein Monoklonaler Antikörper

Alpha Synuclein Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1B10E9

Rezensionen (4)

Kat-Nr. : 66412-1-Ig

Synonyme

Alpha synuclein, a-Synuclein, NACP, PARK1, PARK4, PD1, SNCA

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 66412-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66412-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66412-1-Ig (Alpha Synuclein antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66412-1-Ig (Alpha Synuclein antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66412-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, humanes Gliomgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

66412-1-Ig bindet in WB, IHC, IF, ELISA Alpha Synuclein und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alpha Synuclein fusion protein Ag1285
• Vollständiger Name: synuclein, alpha (non A4 component of amyloid precursor)
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 18-19 kDa
• GenBank-Zugangsnummer: BC013293
• Gene symbol: SNCA
• Gene ID (NCBI): 6622
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.

Publikationen

Species	Application	Title
human	IF	Appl Microbiol Biotechnol; The alteration of intestinal mucosal α-synuclein expression and mucosal microbiota in Parkinson's disease; Authors - Jihua Shi
rat	IHC	Biomed Pharmacother; Nardosinone regulates the slc38a2 gene to alleviate Parkinson's symptoms in rats through the GABAergic synaptic and cAMP pathways.; Authors - Li-Hua Bian
rat	IHC	J Neuropathol Exp Neurol; Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.; Authors - Wenfeng Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Janelle (Verified Customer) (09-12-2022)

Perfect for WB!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

BISHR (Verified Customer) (05-10-2022)

I used it in WB with 1:1000 ration, the band was clear and almost perfect. However, it says the expected kd ranges from 14 to 18, unfortunately I am getting far higher bands up to 50 kd sometimes. Although I am using concentrated acrylamide in the gel, it is making it difficult for reviewers to understand that.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: SH-SY5Y

Alpha Synuclein Antibody Western Blot,Immunofluorescence validation ( dilution) in SH-SY5Y (Cat no:66412-1-Ig)

FH

q (Verified Customer) (01-05-2022)

It is good in WB with a specific band at 17 kd around.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

Alpha Synuclein Antibody Western Blot validation (1:3000 dilution) in mouse brain lysate (Cat no:66412-1-Ig)

FH

Sheela (Verified Customer) (07-16-2019)

This product worked as expected

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse brain lysate

Alpha Synuclein Antibody Western Blot, validation (1:1000 dilution) in Mouse brain lysate (Cat no:66412-1-Ig)