ZO-1 Monoklonaler Antikörper

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• KD/KO Validated

ZO-1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Hund und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

51

CloneNo.

1G4A1

Kat-Nr. : 66452-1-Ig

Synonyme

Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1

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Galerie der Validierungsdaten

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  WB analysis using 66452-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66452-1-Ig (ZO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66452-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66452-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66452-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66452-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66452-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

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  IF Staining of human breast cancer using 66452-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 66452-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MDCK using 66452-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 66452-1-Ig (ZO-1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, A431-Zellen, COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, humanes Mammakarzinomgewebe, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IHC: See 9 publications below
• IF: See 25 publications below

Produktinformation

66452-1-Ig bindet in WB, IHC, IF, ELISA ZO-1 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZO-1 fusion protein Ag16454
• Vollständiger Name: tight junction protein 1 (zona occludens 1)
• Berechnetes Molekulargewicht: 1748 aa, 195 kDa
• Beobachtetes Molekulargewicht: 230 kDa
• GenBank-Zugangsnummer: BC111712
• Gene symbol: TJP1
• Gene ID (NCBI): 7082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).

Publikationen

Species	Application	Title
	WB	Sci Adv; Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage; Authors - Dayun Feng
human	WB,IF	Nat Commun; An electrochemical biosensor for the detection of epithelial-mesenchymal transition.; Authors - Xin Du
human	IF	Cell Death Differ; SARS-CoV-2 spike protein dictates syncytium-mediated lymphocyte elimination.; Authors - Zhengrong Zhang
human	IF	ACS Appl Mater Interfaces; Betaine-Based and Polyguanidine-Inserted Zwitterionic Micelle as a Promising Platform to Conquer the Intestinal Mucosal Barrier; Authors - Kedong Liu
human	IF	Int J Biol Macromol; zwitterionic Pluronic analog-coated PLGA nanoparticles for oral insulin delivery; Authors - Kedong Liu
	IF	Cell Mol Life Sci; Silencing circSERPINE2 restrains mesenchymal stem cell senescence via the YBX3/PCNA/p21 axis; Authors - Fenglei Chen