ZNHIT3 Monoklonaler Antikörper

ZNHIT3 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C7A3

Kat-Nr. : 68331-1-Ig

Synonyme

HNF 4a coactivator, TR interacting protein 3, TRIP 3, TRIP3, zinc finger, HIT type 3, ZNHIT3

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Galerie der Validierungsdaten

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  WB analysis of hTERT-RPE1 using 68331-1-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68331-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 68331-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68331-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ZNHIT3 antibody (68331-1-Ig, Clone: 1C7A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: hTERT-RPE1-Zellen, Jurkat-Zellen, MOLT-4-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68331-1-Ig bindet in WB, IF, ELISA ZNHIT3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZNHIT3 fusion protein Ag29872
• Vollständiger Name: zinc finger, HIT type 3
• Berechnetes Molekulargewicht: 155 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC017931
• Gene symbol: ZNHIT3
• Gene ID (NCBI): 9326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNHIT3, also named TRIP3, belongs to the zinc finger HIT (Zf-HIT) domain-containing proteins family. ZNHIT3 encodes a nuclear zinc finger protein previously implicated in transcriptional regulation and small nucleolar ribonucleoprotein particle assembly and thus possibly to pre-ribosomal RNA processing (PMID: 28335020). ZNHIT3 contains at least two domains: a ZN-HIT domain that consists of a double zinc-finger, probably involved in protein−protein interaction, and a PAC-HIT domain that folds into a clamp able to trap an α-helix, here again of about 20 residues, located in the sequence of NUFIP1 (PMID: 35315277). ZNHIT3 has 2 isoforms produced by alternative splicing.