ZNF259 Polyklonaler Antikörper

ZNF259 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15558-1-AP

Synonyme

zinc finger protein 259, Zinc finger protein ZPR1, ZNF259, ZPR1

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Galerie der Validierungsdaten

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  WB analysis of human brain using 15558-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15558-1-AP (ZNF259 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15558-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15558-1-AP (ZNF259 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 15558-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 15558-1-AP (ZNF259 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15558-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15558-1-AP (ZNF259 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 15558-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 15558-1-AP (ZNF259 Antibody) at dilution of 1:50.

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  IHC staining of human skin using 15558-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15558-1-AP (ZNF259 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 15558-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15558-1-AP (ZNF259 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 15558-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 15558-1-AP (ZNF259 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 15558-1-AP

  Immunofluorescent analysis of HepG2 cells using 15558-1-AP (ZNF259 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, DU 145-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15558-1-AP bindet in WB, IHC, IF, ELISA ZNF259 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF259 fusion protein Ag7980
• Vollständiger Name: zinc finger protein 259
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC004256
• Gene symbol: ZNF259
• Gene ID (NCBI): 8882
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZNF259, also named as Zpr1, is an evolutionarily conserved protein characterized by two C4 zinc fingers and two Zpr1-specific conserved homology domains. Zpr1 was first identified in mammalian cells as a cytoplasmic protein that was capable of binding the intracellular domain of the unactivated EGF receptor (EGFR). The MW of this protein is 51 kDa, and this antibody specially recognises the 51 kDa protein.