ZNF174 Polyklonaler Antikörper

ZNF174 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18157-1-AP

Synonyme

AW 1, zinc finger protein 174, ZNF174, ZSCAN8

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 18157-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18157-1-AP (ZNF174 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18157-1-AP (ZNF174 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 18157-1-AP

  Immunofluorescent analysis of Hela cells, using ZNF174 antibody 18157-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18157-1-AP bindet in WB, IHC, IF, ELISA ZNF174 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZNF174 fusion protein Ag12963
• Vollständiger Name: zinc finger protein 174
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC000876
• Gene symbol: ZNF174
• Gene ID (NCBI): 7727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.