ZHX2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZHX2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 20136-1-AP

Synonyme

AFP regulator 1, AFR1, Alpha fetoprotein regulator 1, KIAA0854, RAF, Regulator of AFP, ZHX2, zinc fingers and homeoboxes 2

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Galerie der Validierungsdaten

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  WB analysis using 20136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20136-1-AP (ZHX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20136-1-AP

  WB result of ZHX2 antibody (20136-1-AP; 1:24000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZHX2 transfected HEK-293 cells.

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  WB analysis of mouse lung using 20136-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20136-1-AP (ZHX2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20136-1-AP (ZHX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20136-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20136-1-AP (ZHX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 20136-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20136-1-AP (ZHX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20136-1-AP

  IP Result of anti-ZHX2 (IP:20136-1-AP, 4ug; Detection:20136-1-AP 1:800) with mouse brain tissue lysate 2800ug.

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  IHC staining of human colon cancer using 20136-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20136-1-AP (ZHX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20136-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20136-1-AP (ZHX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20136-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20136-1-AP (ZHX2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, Maus-Eierstockgewebe, Maushirngewebe, Mauslungengewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

20136-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA ZHX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZHX2 fusion protein Ag14088
• Vollständiger Name: zinc fingers and homeoboxes 2
• Berechnetes Molekulargewicht: 837 aa, 92 kDa
• Beobachtetes Molekulargewicht: 92-100 kDa
• GenBank-Zugangsnummer: BC042145
• Gene symbol: ZHX2
• Gene ID (NCBI): 22882
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

Publikationen

Species	Application	Title
human	WB	Int J Cancer; HBV suppresses ZHX2 expression to promote proliferation of HCC through miR-155 activation.; Authors - Xiaojia Song
human	WB	J Pathol; ZHX2 inhibits SREBP1c-mediated de novo lipogenesis in hepatocellular carcinoma via miR-24-3p.; Authors - Xiangguo Yu
mouse	WB	J Immunol; Zhx2 Accelerates Sepsis by Promoting Macrophage Glycolysis via Pfkfb3.; Authors - Zehua Wang ; KO Validated
human	WB,IHC	Cancer Cell Int; ZHX2 inhibits thyroid cancer metastasis through transcriptional inhibition of S100A14.; Authors - Yankun Zhang ; KD Validated
human	WB	Biochem Biophys Res Commun; Proliferation and osteo/odontogenic differentiation of stem cells from apical papilla regulated by Zinc fingers and homeoboxes 2: An in vitro study.; Authors - Fang Wan ; KD Validated
human	WB,IHC	FEBS Open Bio; Upregulation of ZHX2 predicts poor prognosis and is correlated with immune infiltration in gastric cancer.; Authors - Anqi Cheng