ZG16 Polyklonaler Antikörper

ZG16 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 17397-1-AP

Synonyme

hZG16, JCLN, JCLN1, Secretory lectin ZG16, ZG16, ZG16A, Zymogen granule protein 16

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Galerie der Validierungsdaten

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  WB analysis of human colon using 17397-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 17397-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17397-1-AP

  mouse colon tissue lysates were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17397-1-AP (ZG16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 17397-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17397-1-AP (ZG16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse small intestine using 17397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (17397-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolongewebe, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe, humanes Pankreasgewebe, Maus-Dünndarmgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:600
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

17397-1-AP bindet in WB, IHC, IF, ELISA ZG16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZG16 fusion protein Ag11332
• Vollständiger Name: zymogen granule protein 16 homolog (rat)
• Berechnetes Molekulargewicht: 167 aa, 18 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC029149
• Gene symbol: ZG16
• Gene ID (NCBI): 653808
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; Co-transplantation of autologous Treg cells in a cell therapy for Parkinson's disease; Authors - Tae-Yoon Park