ZFPM1 Polyklonaler Antikörper

ZFPM1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28012-1-AP

Synonyme

FOG, FOG 1, FOG1, Friend of GATA 1, Friend of GATA protein 1, ZFPM1, Zinc finger protein ZFPM1, ZNF408, ZNF89A

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 28012-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 28012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 28012-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 28012-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28012-1-AP bindet in WB, IHC, ELISA ZFPM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZFPM1 fusion protein Ag27829
• Vollständiger Name: zinc finger protein, multitype 1
• Berechnetes Molekulargewicht: 105 aa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: NM_153813
• Gene symbol: ZFPM1
• Gene ID (NCBI): 161882
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZFPM1 (Zinc Finger Protein, FOG Family Member 1, previous known as FOG1) is a protein with nine zinc finger domains, the C2HC type zinc fingers on ZFPM1 protein are responsible for mediating the interaction with the N-finger of GATA factors (PMID:10329627). ZFPM1 also contains two domains that are known to recruit transcriptional regulatory complexes. The one located at the extreme N-terminus is essential to recruit the nucleosome remodeling and histone deacetylase (NuRD) complex, another one between zinc fingers 6 and 7 could bind to CtBP co-repressor (PMID:30611731).