HIP14; ZDHHC17 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HIP14; ZDHHC17 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15465-1-AP

Synonyme

DHHC 17, HIP 14, HIP 3, HIP14, HIP14; ZDHHC17, HIP3, HSPC294, Huntingtin yeast partner H, HYPH, KIAA0946, Palmitoyltransferase ZDHHC17, ZDHHC17

---

Galerie der Validierungsdaten

• 

  WB analysis of Raji using 15465-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 15465-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 15465-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15465-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15465-1-AP bindet in WB, IHC, IF, ELISA HIP14; ZDHHC17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HIP14; ZDHHC17 fusion protein Ag7763
• Vollständiger Name: zinc finger, DHHC-type containing 17
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC050324
• Gene symbol: ZDHHC17
• Gene ID (NCBI): 23390
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.

Publikationen

Species	Application	Title
human	IF, WB	J Cell Biol; Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.; Authors - Thaddaeus Mutugi Nthiga
human	WB	mBio; Identification of ZDHHC17 as a Potential Drug Target for Swine Acute Diarrhea Syndrome Coronavirus Infection; Authors - Yun Luo ; KO Validated
	WB	J Cell Sci; Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus; Authors - Danya Abazari
mouse	IHC	Mol Cell Endocrinol; ZDHHC17 participates in the pathogenesis of polycystic ovary syndrome by affecting androgen conversion to estrogen in granulosa cells; Authors - Shanmeizi Zhao