ZCRB1 Polyklonaler Antikörper

ZCRB1 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 25629-1-AP

Synonyme

MADP 1, MADP1, RBM36, U11/U12 31K, U11/U12 snRNP 31 kDa protein, ZCCHC19, ZCRB1

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Galerie der Validierungsdaten

• 

  WB analysis of mouse testis using 25629-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 25629-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25629-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 25629-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 25629-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25629-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 25629-1-AP

  Immunofluorescent analysis of HepG2 cells using 25629-1-AP (ZCRB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Neuro-2a-Zellen, Raji-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25629-1-AP bindet in WB, IF, ELISA ZCRB1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZCRB1 fusion protein Ag22095
• Vollständiger Name: zinc finger CCHC-type and RNA binding motif 1
• Berechnetes Molekulargewicht: 217 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC022543
• Gene symbol: ZCRB1
• Gene ID (NCBI): 85437
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; A novel protein encoded by ZCRB1-induced circHEATR5B suppresses aerobic glycolysis of GBM through phosphorylation of JMJD5.; Authors - Jian Song