ZCCHC8 Polyklonaler Antikörper

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• KD/KO Validated

ZCCHC8 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 23374-1-AP

Synonyme

ZCCHC8

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Galerie der Validierungsdaten

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  WB analysis using 23374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23374-1-AP (ZCCHC8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 23374-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 23374-1-AP (ZCCHC8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 23374-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 23374-1-AP (ZCCHC8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23374-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23374-1-AP (ZCCHC8 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 23374-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23374-1-AP (ZCCHC8 antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HepG2 using 23374-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23374-1-AP (ZCCHC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

23374-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ZCCHC8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZCCHC8 fusion protein Ag19985
• Vollständiger Name: zinc finger, CCHC domain containing 8
• Berechnetes Molekulargewicht: 707 aa, 79 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC065918
• Gene symbol: ZCCHC8
• Gene ID (NCBI): 55596
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. ZCCHC8 acts as a scaffold, mediating the interaction between RBM7 and MTR4. NEXT is involved in exosome-mediated surveillance and decay of noncoding RNAs, such as enhancer RNAs (eRNAs) and aberrant 3′-extended transcripts from small nuclear RNA (snRNA), telomerase RNA, and replication-dependent histone genes. NEXT is an important node in regulating nuclear exosome activities and takes part in several biological processes, including DNA damage response, stress response, and viral ribogenesis . ZCCHC8 also has a robust interaction with YTHDC1 (PMID: 28984244).

Publikationen

Species	Application	Title
mouse	WB,IP	Mol Cell; Nuclear RNA catabolism controls endogenous retroviruses, gene expression asymmetry, and dedifferentiation; Authors - Denis Torre ; KD Validated
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan
human	CoIP	Nucleic Acids Res; Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2.; Authors - Linlin Hou
mouse	WB	Cell Rep; Nuclear Exosome Targeting Complex Core Factor Zcchc8 Regulates the Degradation of LINE1 RNA in Early Embryos and Embryonic Stem Cells.; Authors - You Wu ; KO Validated
human	WB	Nat Neurosci; Globally reduced N6-methyladenosine (m6A) in C9ORF72-ALS/FTD dysregulates RNA metabolism and contributes to neurodegeneration; Authors - Yini Li ; KD Validated
human	WB	PLoS Genet; RBMX involves in telomere stability maintenance by regulating TERRA expression; Authors - Jingfan Liu