ZC3H12A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZC3H12A Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 25009-1-AP

Synonyme

MCP induced protein 1, MCPIP, MCPIP1, Ribonuclease ZC3H12A, RP3 423B22.1, ZC3H12A

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 25009-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 25009-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 25009-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 25009-1-AP

  IP Result of anti-ZC3H12A (IP:25009-1-AP, 4ug; Detection:25009-1-AP 1:300) with Raji cells lysate 2000ug.

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  IHC staining of human small intestine using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25009-1-AP (ZC3H12A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 25009-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25009-1-AP (ZC3H12A Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Mausmilzgewebe, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

25009-1-AP bindet in WB, IP, IHC, IF, ELISA ZC3H12A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZC3H12A fusion protein Ag13877
• Vollständiger Name: zinc finger CCCH-type containing 12A
• Berechnetes Molekulargewicht: 599 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC005001
• Gene symbol: ZC3H12A
• Gene ID (NCBI): 80149
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.

Publikationen

Species	Application	Title
mouse	WB	J Cell Physiol; MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR-155.; Authors - Zhenzhen Li ; KD Validated
mouse	WB	Cell Physiol Biochem; Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6.; Authors - Zhenzhen Li
mouse	IHC	J Immunol; MCPIP1/Regnase-1 Restricts IL-17A- and IL-17C-Dependent Skin Inflammation.; Authors - Leticia Monin
	WB	Retrovirology; Increased expression of CDKN1A/p21 in HIV-1 controllers is correlated with upregulation of ZC3H12A/MCPIP1.; Authors - Suwellen S D de Azevedo
mouse	IF	Neurosci Lett; TLR4 signaling pathway mediates the LPS/ischemia-induced expression of monocytechemotactic protein-induced protein 1 in microglia.; Authors - Shumin Chen
mouse	IF	Med Microbiol Immunol; MCPIP1 inhibits coxsackievirus B3 replication by targeting viral RNA and negatively regulates virus-induced inflammation.; Authors - Min Li