YWHAH Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

YWHAH Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 15222-1-AP

Synonyme

14 3 3 protein eta, 14-3-3, 14-3-3 eta, Protein AS1, YWHA1, YWHAH

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Galerie der Validierungsdaten

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  WB analysis of human brain using 15222-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15222-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15222-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15222-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15222-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 15222-1-AP

  IP Result of anti-YWHAH (IP:15222-1-AP, 4ug; Detection:15222-1-AP 1:1000) with NIH/3T3 cells lysate 1600ug.

• 

  IF Staining of HeLa using 15222-1-AP

  Immunofluorescent analysis of Hela cells, using YWHAH antibody 15222-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, A431-Zellen, Maushirngewebe, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15222-1-AP bindet in WB, IP, IF, ELISA YWHAH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YWHAH fusion protein Ag7435
• Vollständiger Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC003047
• Gene symbol: YWHAH
• Gene ID (NCBI): 7533
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YWHAH (also known as 14-3-3 eta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAH.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; Circulating Exosomal MiR-107 Restrains Tumorigenesis in Diffuse Large B-Cell Lymphoma by Targeting 14-3-3η.; Authors - Jiarui Liu
rat	WB	Eur J Pharmacol; Capsaicin prevents mitochondrial damage, protects cardiomyocytes subjected to anoxia/reoxygenation injury mediated by 14-3-3η/Bcl-2.; Authors - Jiyi Huang
human,mouse	WB,IF,IP	J Exp Clin Cancer Res; 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.; Authors - Jia Song ; KD Validated
rat	WB	Cells; Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development; Authors - Nghi M Nguyen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vicki (Verified Customer) (01-13-2023)

Very expressive antibody used on BDEVs

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: EVs from Rat Brain

YWHAH Antibody Western Blot validation (1:2000 dilution) in EVs from Rat Brain (Cat no:15222-1-AP)

FH

Vicki (Verified Customer) (09-12-2022)

Works extremely well on hard to develop samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: EVs from Rat Brain

YWHAH Antibody Western Blot validation (1:1000 dilution) in EVs from Rat Brain (Cat no:15222-1-AP)