YTHDC1 Monoklonaler Antikörper

YTHDC1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F5G11

Rezensionen (2)

Kat-Nr. : 67911-1-Ig

Synonyme

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

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Galerie der Validierungsdaten

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  WB analysis using 67911-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67911-1-Ig (YTHDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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  IHC staining of human breast cancer using 67911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67911-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 67911-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 67911-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67911-1-Ig bindet in WB, IHC, ELISA YTHDC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: YTHDC1 fusion protein Ag6289
• Vollständiger Name: YTH domain containing 1
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: ~100 kDa
• GenBank-Zugangsnummer: BC053863
• Gene symbol: YTHDC1
• Gene ID (NCBI): 91746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

Good quality with clear band.

• Applications: Western Blot
• Cell Tissue Type: HEK293T

FH

Xingyuan (Verified Customer) (06-30-2023)

Quality was good. Did show clear band for YTHDC1 at around 100kDa for the western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: 293T

YTHDC1 Antibody Western Blot validation (1:4000 dilution) in 293T (Cat no:67911-1-Ig)