YTHDC1 Polyklonaler Antikörper

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• KD/KO Validated

YTHDC1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

26

Kat-Nr. : 14392-1-AP

Synonyme

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 14392-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14392-1-AP (YTHDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14392-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14392-1-AP (YTHDC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse tissue using 14392-1-AP

  WB result of YTHDC1 (14392-1-AP) antibody with mouse sample.

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  IHC staining of mouse brain using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 5 publications below

Produktinformation

14392-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ELISA YTHDC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YTHDC1 fusion protein Ag5882
• Vollständiger Name: YTH domain containing 1
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: ~100 kDa
• GenBank-Zugangsnummer: BC053863
• Gene symbol: YTHDC1
• Gene ID (NCBI): 91746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDC1, also named as KIAA1966 or YT521, is a 727 animo acid protein, which contains 1 YTH domain. YTHDC1 localizes in the nucleus and Interacts with EMD, KHDRBS1/SAM68, KHDRBS3, TRA2B/SFRS10, SC35 and SF2. YTHDC1 as a RNA-binding protein that regulates alternative splice site selection. The calcualted molecular weight of YTHDC1 is 85 kDa, but modified TFEB is about 110 kDa (PMID: 10973987 ).

Publikationen

Species	Application	Title
mouse	WB	Nature; m6A facilitates hippocampus-dependent learning and memory through YTHDF1.; Authors - Hailing Shi
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen
human	WB,IF	Nucleic Acids Res; N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.; Authors - Haojie Hao
human	WB	Cell Death Differ; YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect.; Authors - Yichao Hou
mouse	RIP	Dev Cell; N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.; Authors - Jie Du
human	WB,IP	Mol Ther Nucleic Acids; METTL14-mediated Lnc-LSG1 m6A modification inhibits clear cell renal cell carcinoma metastasis via regulating ESRP2 ubiquitination.; Authors - Danyang Shen ; KD Validated