YPEL3 Polyklonaler Antikörper

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• KD/KO Validated

YPEL3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 15403-1-AP

Synonyme

Protein yippee like 3, yippee like 3 (Drosophila), YPEL3

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 15403-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15403-1-AP (YPEL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 15403-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15403-1-AP (YPEL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 15403-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15403-1-AP (YPEL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15403-1-AP bindet in WB, IHC, IF, ELISA YPEL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YPEL3 fusion protein Ag7626
• Vollständiger Name: yippee-like 3 (Drosophila)
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC005009
• Gene symbol: YPEL3
• Gene ID (NCBI): 83719
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YPEL3 is part of a five-member family of closely related paralogues: YPEL1-5, which are named in reference to their Drosophila ortholgue. Studies showed shat YPEL3 is regulated by p53 and its encoding protein induces cellular senescence in human tumor and normal cells, indicating that YPEL3 is a p53-dependent, tumor suppressor gene. (PMID:20388804) 15403-1-AP was raised against full length of YPEL3 protein (1-119aa); it can recognize YPEL1-4.

Publikationen

Species	Application	Title
human	WB	Cancer Res; YPEL3, a p53-regulated gene that induces cellular senescence.; Authors - Kelley Kevin D KD ; KD Validated
human	WB	Int J Cancer; Novel senescence associated gene, YPEL3, is repressed by estrogen in ER+ mammary tumor cells and required for tamoxifen-induced cellular senescence.; Authors - Tuttle Rebecca R
human	WB,IF	Sci Rep; Steroid sulfatase deficiency causes cellular senescence and abnormal differentiation by inducing Yippee-like 3 expression in human keratinocytes.; Authors - Hyoung-Seok Baek
human	IHC	Front Genet; Identification of a cellular senescence-related-lncRNA (SRlncRNA) signature to predict the overall survival of glioma patients and the tumor immune microenvironment; Authors - Qing Liu