YAP1 Monoklonaler Antikörper

YAP1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen

2

CloneNo.

3A7A9

Kat-Nr. : CL488-66900

Synonyme

65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog

---

Galerie der Validierungsdaten

• 

  IF Staining of HepG2 using CL488-66900

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66900 (YAP1 antibody), at dilution of 1:100.

• 

  IF Staining of HepG2 using CL488-66900

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 YAP1 antibody (CL488-66900, Clone: 3A7A9 ) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL488-66900

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66900 (YAP1 antibody) at dilution of 1:100.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:10-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

CL488-66900 bindet in WB, IF YAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: YAP1 fusion protein Ag28194
• Vollständiger Name: Yes-associated protein 1, 65kDa
• Berechnetes Molekulargewicht: 504 aa, 54 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC038235
• Gene symbol: YAP1
• Gene ID (NCBI): 10413
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001).The calcualted molecular weight of YAP1is 54 kDa, but phosphorylated YAP1 is about 65-70 kDa. (PMID: 26695440)

Publikationen

Species	Application	Title
mouse	WB	Glia; Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes; Authors - Yue Wan
human	IF	Biomedicines; Inactivation of Yes-Associated Protein Mediates Trophoblast Dysfunction: A New Mechanism of Pregnancy Loss Associated with Anti-Phospholipid Antibodies?; Authors - Zengshu Huang