XRCC4 Polyklonaler Antikörper

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• KD/KO Validated

XRCC4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 15817-1-AP

Synonyme

DNA repair protein XRCC4, XRCC4

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15817-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP (XRCC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15817-1-AP

  WB result of XRCC4 antibody (15817-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XRCC4 transfected HEK-293 cells.

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  WB analysis of HepG2 using 15817-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP (XRCC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15817-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP (XRCC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15817-1-AP

  IP Result of anti-XRCC4 (IP:15817-1-AP, 4ug; Detection:15817-1-AP 1:800) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human breast cancer using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15817-1-AP (XRCC4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15817-1-AP (XRCC4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of rat small intestine using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of rat testis using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of rat testis using 15817-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Ratten-Dünndarmgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below

Produktinformation

15817-1-AP bindet in WB, IP, IHC, ELISA XRCC4 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XRCC4 fusion protein Ag8606
• Vollständiger Name: X-ray repair complementing defective repair in Chinese hamster cells 4
• Berechnetes Molekulargewicht: 310 aa, 35 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC005259
• Gene symbol: XRCC4
• Gene ID (NCBI): 7518
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.; Authors - Guijie Guo ; KD Validated
human	WB	Cancer Res; Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining.; Authors - Qin Zhou
human	WB	Cell Death Differ; LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair.; Authors - Dan Wang
human	WB	Toxicol Appl Pharmacol; Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.; Authors - Hui Dong
human	WB	PLoS One; 5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation.; Authors - Wei Jiang
human	WB	J Cancer; Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma.; Authors - Yi Liu