XRCC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

XRCC1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 21468-1-AP

Synonyme

DNA repair protein XRCC1, RCC, XRCC1

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Galerie der Validierungsdaten

• 

  WB analysis using 21468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21468-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21468-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21468-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21468-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21468-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21468-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21468-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21468-1-AP (XRCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 21468-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 21468-1-AP (XRCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

21468-1-AP bindet in WB, IHC, IF, ELISA XRCC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XRCC1 fusion protein Ag15172
• Vollständiger Name: X-ray repair complementing defective repair in Chinese hamster cells 1
• Berechnetes Molekulargewicht: 633 aa, 70 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC023593
• Gene symbol: XRCC1
• Gene ID (NCBI): 7515
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.; Authors - Mingming Yang
human	WB	Mol Ther; PMID: 29456019; Authors - Xinyi Wang
human	WB	J Cell Mol Med; Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.; Authors - Xingjie Gao
human	WB	Int J Environ Res Public Health; Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM2.5 from Beijing, China, in Winter.; Authors - Bing-Yu Niu
human	WB	Toxicol Appl Pharmacol; Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.; Authors - Hui Dong
human	WB,IHC	Front Mol Biosci; XRCC1 Is a Promising Predictive Biomarker and Facilitates Chemo-Resistance in Gallbladder Cancer.; Authors - Zhengchun Wu ; KD Validated