XPNPEP3 Polyklonaler Antikörper

XPNPEP3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15655-1-AP

Synonyme

Aminopeptidase P3, APP3, APP3c, APP3m, X Pro aminopeptidase 3, XPNPEP3

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Galerie der Validierungsdaten

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  WB analysis using 15655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15655-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 15655-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 15655-1-AP

  IP Result of anti-XPNPEP3 (IP:15655-1-AP, 4ug; Detection:15655-1-AP 1:400) with PC-3 cells lysate 2000ug.

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  IHC staining of human lung cancer using 15655-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15655-1-AP (XPNPEP3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 15655-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15655-1-AP (XPNPEP3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human oesophagus using 15655-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 15655-1-AP (XPNPEP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 15655-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 15655-1-AP (XPNPEP3 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, 4T1-Zellen, humanes Hodengewebe, humanes Milzgewebe, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Ösophagusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15655-1-AP bindet in WB, IP, IHC, ELISA XPNPEP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XPNPEP3 fusion protein Ag8007
• Vollständiger Name: X-prolyl aminopeptidase (aminopeptidase P) 3, putative
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC004989
• Gene symbol: XPNPEP3
• Gene ID (NCBI): 63929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XPNPEP3 gene encodes X-prolyl aminopeptidase 3 which functions to remove the penultimate N-terminal Proline residue from nascent proteins and appears to play a role in ciliary function. CRC tissue microarray revealed increased XPNPEP3 expression in tumor compared to matched normal samples (PMID:29383790). Other study found that XPNPEP3 localizes to mitochondria in renal cells in vitro and to kidney tubules in a cell type-specific pattern, and mutations in the gene are associated with NPHP-like disorder (PMID:20179356).